DETECTION OF TERNARY COMPLEX OF FIBRIN DESAB WITH D-DIMER AND D-FRAGMENT OF FIBRIN

The aim of this work is to study the intermolecular interactions fibrin with D-domain-containing fragments fibrin(ogen): D-dimer and D-fragment. Materials methods. Human fibrinogen was obtained from human blood plasma by salt extraction using 16 % Na2SO4. content protein coagulated thrombin – 96-98%. Analytical size-exclusion chromatography for detection molecular complexes performed on Sepharose 6B column (30 x 0.5 cm). Components analyzed mixture (0.8 ml) were separated standard protocol: speed elution ml/min; collected samples volume ml. Optical density measured spectrophotometer POP (Optizen, Daejeon, Korea). Composition each sample SDS-PAGE. Relative amounts studied compounds in densitometry scanned electropherograms Totallab TL100 software. Molecular modeling formed desAB its UCSF Chimera 1.16 basis earlier developed protofibril structure. structure D-region (PDB ID:1LTJ) prepared same in-protein docking HDOCK web server. Results. To investigate complex formation between desAB. appearance D- DD-fragments zone 5.5 mL, which does not overlap individual (7.5-9.5 mL), detected, indicating a ternary complex. Densitometry TotalLab TL-100 demonstrated that average densities pixels bands desAB, D-fragment equal. It means composed approximate ratio 1:1:1. software used establish spatial arrangement relation molecule bound D-dimer. Conclusions. We characterized followed Further properties may clarify certain issues related polymerization, namely process their branching.